About the role
Associate Scientist III, wanted by Insitro, Inc. in South San Francisco, CA. Onboarding new cell models and developing quality control metrics for automated high-throughput arrayed genetic and compounding cell-screening assays suitable for downstream machine learning based discovery; Preparing, optimizing, and implementing real-time qPCR assays to support comprehensive target validation screenings by measuring knockdown efficiency, generating dose-response curves, and screening compound inhibitors. Applying advanced perturbation techniques—such as RNA interference, CRISPR-based gene editing, and small molecule modulation—to ensure assay fidelity, confirm target engagement, and generate robust, precise data for evaluating gene silencing effectiveness and compound efficacy; Leveraging on-flight QC and advanced Python-based data analysis to systematically assess assay performance, ensuring data quality and reproducibility for high-throughput screening, and driving insights into new assay development. Independently troubleshoot and characterize assay performance issues, optimizing key parameters and informing critical decisions. Present findings and data-driven recommendations to cross-functional teams to facilitate strategic improvements in assay design and execution; Developing confocal imaging experiments that result in high-content imaging data and perform in-house image analyses for assay characterization; In time metadata registration and maintaining thorough documentation within electronic lab notebooks (ELNs), ensuring data traceability and compliance. Leading the development and continuous improvement of standard operating procedures (SOPs), while upholding all laboratory safety protocols and training standards to support operational excellence; Designing and developing innovative assay readout panels, incorporating advanced techniques and methodologies to enhance the sensitivity, accuracy, and scalability of screening assays. Ensuring the new panels meet the specific requirements of ongoing research and facilitating high-quality data generation.
Must have a Master’s degree in Biotechnology, or a related field plus two (2) years of experience in a related occupation. In lieu of a Master’s degree plus two (2) years of experience, we will accept a Bachelor’s degree in Biotechnology, or a related field plus five (5) years of progressive experience in a related occupation. Must have two (2) of years experience in designing and conducting hypothesis-driven experiments including constructing statistical models for Design of Experiment based analysis and conducting data driven analysis of point of failures in experiments using Python; two (2) years experience of medium/high-throughput cell-based screening assays (96/384-well format) for phenotype screening or compound screening on human stems cells, iPSC cells or primary cells; two (2) years of experience executing cell screening assays including developing fluorescence- and absorbance-based assays using instruments such as the Tecan Spark or high-content confocal imaging Opera Phenix Plus HCS system; creating protocols for automated liquid handling systems including the Agilent BioTek EL406 and HAMILTON platforms; and performing QC metrics and performing data analysis to validate assay reproducibility and quality; two (2) years of experience using molecular biology techniques, including PCR, qPCR, and gel electrophoresis. This includes performing DNA and RNA extractions using Qiagen kits, running qPCR on the QuantStudio system, designing experiments, preparing samples with custom probes, analyzing data outputs, and evaluating the effects of drug treatments; and two (2) years of experience culturing human stem cells, iPSC cells, or primary cells. $126,000-$129,000/yr. Please send resume to: ta-resumes@insitro.com and refer to: Job Req.2026-insitro-004P